Analysis Note

ControlType II astrocytes, human neural progenitors

Application

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Detect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH.

Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.

Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).

May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.

May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.

Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.

Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)

Complement-mediated cytotoxicity (Eisenbarth et al., 1979)

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Embryonic chicken retinal cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.

Format: Purified

Specificity

Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.